“The Two R’s: A Paradigm Shift in Beekeeping”

By

Dr. Malcolm T. Sanford
http://apis.shorturl.com

 

By the time you read this, my good friend Laurence Cutts will have retired as Florida’s Chief bee inspector.  August 28, 2003 will bring to an end one of the longest-running tenures of a chief apiarist in the Sunshine state.  The Florida beekeeping industry will be losing an official who by all accounts was responsible for getting the industry through some of its most challenging times.  This statement was confirmed in a recent Florida Honey Bee Technical Council meeting by one of the state’s largest beekeepers.  Just as Laurence came on the bee inspection scene, the tracheal mite invasion of 1984 was in full tilt.  Hard on its heels, Varroa mites were discovered in 1987.  Laurence, first and foremost a beekeeper, saw the industry that had been on the no-pesticide-application-for-farmers side of the fence, tear it down to get to the other side.  Like all of us, he was shocked at how quickly the industry adroitly stepped on to a pesticide treadmill of its own that continues to this day. 

Africanized honey bees came to Texas and went west after 1990.  There has been no colonization of this bee in Florida, only sporadic finds of swarms off ships.  Laurence often jokingly takes credit for the fact that the bee has not spread east from the Lone Star state, but it is to his credit that incipient invasions from Pensacola to Tampa have been nipped in the bud by an aggressive trapping system run by his dedicated inspection crew.

Laurence has been a champion for Florida beekeepers obtaining chemical treatments for their bees as the mite then beetle scourges appeared.  He helped push through registration of Apistan® (fluvalinate) and Miticure® (amitraz), only to see the latter material summarily removed from the market due to lawsuits by the beekeeping industry.  When mite resistance to fluvalinate began to appear, he was again at the forefront to obtain the emergency section 18s necessary to begin to use the alternative material coumaphos (CheckMite+®).  He has been successful in getting these renewed as well, something EPA is loath to do for this kind of label.  Again he saw much of his efforts evaporate as the resilient mite population acquired and continues to develop resistance to this class of potent nerve toxins (organophosphates).   He now is involved in helping the registration of the soft chemical mixture called ApiLife Var®. 

If all that above wasn’t enough, the appearance of antibiotic-resistant American foulbrood is now occurring on his watch.  Finally, he was faced with introduction of the small hive beetle, Aethina tumida in 1998.  Laurence came through all these crises with a mixture of confidence and humor that provided a clearing for other beekeepers to do the same.  One of his most important skills is the ability to boil circumstances down to a few pithy words that call to his colleague beekeepers.  His latest pronouncement is the subject of this column, that the two R’s of beekeeping: Resistance and Residues now constitute a paradigm shift in the craft.

The history of mite resistance began in Europe where populations first became resistant to fluvalinate in Italy. Later the phenomenon was discovered in the United States. Because of this, the organophosphate coumaphos (Check Mite +® ) has received several Section 18 registrations for use in cases where the mites are resistant to pyrethroids like fluvalinate.  The status of worldwide resistance to miticides  was recently reported in the minutes of the 5th meeting of the European Group for Integrated Varroa Control.1

The appearance of resistance to both miticides and antibiotics looks to be yet another example of Garrett Hardin’s "Tragedy of the Commons."2  Dr. Marjorie Hoy, eminent scholar - Davies, Fischer and Eckes professor of biological control, of the Department of Entomology and Nematology here at the University of Florida, has given a number of seminars on the development of the phenomenon, the source of much of the information provided here. She says it is strictly a matter of the number of applications, the residue length, and the genetics of the pest, and is the collective fault of both the companies producing pesticides and consumers using them. . Her conclusion is that the only truly effective way to prevent resistance is to stop using pesticides.

If one looks at other examples of resistance to pesticides in production agriculture, it becomes more obvious why this occurred for Varroa. Perhaps the most important factor is prolonged exposure to one pesticide. Certainly this was the case with Apistan®, the only legal, effective material applied for over a decade to many generations of mites. Selection pressure was also very high. Apistan® may have been too good in restrospect. It killed off over 98 percent of the mites, but didn’t get them all. Those left over became a potent source for a resistant population to emerge. The idea that bee populations must be cleared totally of Varroa also contributed greatly. In the beginning and continuing to this day, the concept of Varroa eradication (i.e. "Varroa-free") persists. As a result, many beekeepers treat when they see only one mite; some leave treatments in over prolonged periods and others may apply the material prophylactically, just like treating for American foulbrood using Terramycin®, which most believe resulted in a resistant causative organism. Other factors that contributed were strong links between Varroa and its host (they feed and reproduce only on honey bees). The ability to be carried on the backs of flying bees from one colony to another adds a significant re infestation dimension.

Factors responsible for development of resistance to pesticides, according to Dr. Hoy, are generally grouped into two major categories: controlled and uncontrolled. Unfortunately, most fall into the latter category. In general, these are genetic, biological and ecological. The mite’s genetics and biology remain much of an enigma. Its ecology does too.  This kind of basic study, unfortunately, is often subrogated to pesticide development and application.

It is not surprising that most research and funding in Varroa control has concentrated on toxicological work. This is the one area where the beekeeper and researcher have the best and easiest control. In this kind of work, formulations and applications can be actively tweaked and their effectiveness quickly measured. It is also where the money is. There is little incentive on the part of entrepreneurs to invest in other areas. This is the case with natural products or processes, which although they may be effective, do not represent the best possible return on an economic investment. Again much of this is also true in other forms of production agriculture. Indeed, the loss of legally registered chemicals in small crops not warranting huge economic investments continues to be a crisis all across the agricultural spectrum.

According to Dr. Hoy, researchers have developed models of pesticide resistance where the phenomenon has been detected. However, debate continues about how effective each is. Apiculture appears to have gone through several types on its way to its present situation. Saturation uses high doses of chemicals in an attempt to overcome any resistance that might be present. This is often employed for high-value crops like apples. Synergists have even been used to make already-toxic materials more poisonous. These are very hard on non target organisms and the environment. However, in many systems of this nature resistance appears.

Much of this is applicable for Varroa. In the beginning, low treatment thresholds, the idea of "mite-free colonies," meant that a lot of treatment was probably carried out that was not absolutely necessary. The now-illegal use of Maverik®-soaked wooden strips, originally carrying a Section 18 label, is an example of saturation, as is reported use of other non registered materials (e.g. amitraz formulated in Ovicin®).3

Another strategy is moderation. Its philosophy is to reduce the selective pressure and conserve organisms (genes) that are susceptible. This means less frequent applications with relatively low dosages of less toxic chemicals. Some beekeepers are moving in this direction, applying materials only when absolutely necessary and using less than the recommended dosages. Unfortunately, there is controversy in this arena, with some investigators suggesting that underdosing actually contributes to resistance development. This points to the fact that the genetic cause of resistance is often unknown. In addition, the lack of suitable action threshold levels for Varroa works against this concept .

Multiple attack appears to be the model beekeeping is now leaning toward. This is characterized as a rotational philosophy involving several pesticides. Again this has been employed in other high-value crops and some medical emergencies. Whether to rotate or mix chemicals is vexing, according to Dr. Hoy. Mixing might enable them to be used at lower dosages. Beekeepers now find themselves with two legal materials. Whether to choose mixing or rotating, however, is a question that cannot easily be answered in this case because there is not enough information about the mechanism of resistance by Varroa. Because two classes of pesticides (pyrethroids and organophosphates) are being employed, however, rotation is considered the best strategy and beekeepers are asked not to use both materials simultaneously.

Another problem found in production agriculture is that after pesticide use, pest populations may rebound to higher levels than before. This resurgence is often the result of killing off natural enemies of the pest species during the application process. To date, no such organisms have been found for Varroa and this does not appear to be an issue. Finally, replacement can occur in many systems. Organisms that are not a problem or pest may become one as a consequence of trying to control another pest species. The specter of the small hive beetle for beekeeping is raised here. Is it possible that the beetle has been around a longer time than thought and only showed itself as problematic after colonies were exposed to chronic dosages of pesticides for Varroa control.  I have heard a number of talks where both researchers and regulators say that controlling hive beetles when Varroa is not first controlled is a recipe for disaster.

Other strategies to reduce pesticides include the use of smoke. Other so-called soft chemicals such as natural acids, essential oils or botanicals are also a possibility. Although a few of these "natural products" have been shown to be somewhat effective, they do not kill the number of mites as do registered hard pesticides. However, resistance to these materials may not prove to be as problematic. Methods in applying such chemicals, however, have not been worked out, are full of risk for bees and the action thresholds are unknown.  It is useful to remember that most natural products or biopesticides are poisonous compounds that plants have made to ensure their consumption more risky and less likely. Their use may also be construed as illegal or non registered materials by regulatory authorities, and they are capable of contaminating a honey crop.

In the end, as Dr. Hoy recommends, only by reducing use of pesticides can beekeepers come to grips with either effects of sub lethal pesticide doses or resistance. This is the stated goal of integrated pest management (IPM), a technology that relies on a careful estimation of the pest population. The number of insects (or mites) causing measurable economic loss to a crop (or bee colony) is referred to as the economic injury level (EIL). IPM practitioners attempt to find a level of pest population that will provide the best indication that economic injury is likely to occur. At that point, and only at that point, are active measures (e.g. pesticide application) taken to reduce the pest population. This is called the economic threshold (ET). For example, in a bean crop it might be determined that only when there is a certain amount of leaf damage, which translates into a specific number of caterpillars on a plant, is pesticide application necessary. A key element here is the realization that some pest population will always be present in beans and that economic production can be supported at some level without treatment. A corollary is that eradication is impossible and should be dismissed as a possibility.

There are other ways besides pesticides to keep the mite populations below damaging levels. These include trapping them in drone brood, on sticky boards and by specially designed bottom boards. Another strategy is to identify Varroa-tolerant honey bees and then propagate them. This is currently being done through the Baton Rouge Bee Laboratory’s release of Russian bees and development of SMR stock.

 Most of the control methods suggested above are active. They are applied or exerted by the beekeeper. However, another group should not be forgotten. These are referred to as passive, or collectively called "good bee management." With all the folderol about mites, and now the small hive beetle, the fact that honey bees can often take adequate care of themselves is easily overlooked.

Resistance runs in the company of residues according to Laurence Cutts.  One of the first responses to resistance, common in past agricultural practice, is applying more and more chemical control as the problems increase.  This no doubt has occurred in beekeeping operations.  Although it is not well known why, the appearance of chloramphenicol in Chinese honey may be a response to resistance by American foulbrood to oxytetracycline (Terramycin®).  Study in Europe reveals levels of acaracide residues in honey, beeswax and propolis.  They can only be described as extensive.4 

Certainly, the continued application of both fluvalinate and now coumaphos hastens an end to the era that Laurence Cutts characterizes as that of the “silver bullets”of mite control.  Thus, he gracefully exits the stage as an advocate for Integrated Pest Management (IPM), carrying the message that the use of this technology, developed by dedicated researchers like Dr. Hoy has the best chance at present to keep the two R’s of beekeeping at bay.

References:

1.      5th meeting of the integrated group for Varroa control, June 2000, World Wide Web site accessed August 25, 2003 <http://www.apis.admin.ch/english/host/pdf/alternativ/bern.pdf>.

2.      World Wide Web site accessed August 25, 2003 <http://members.aol.com/trajcom/private/trajcom.htm>.

3.      Elzen, P. J., Baxter, J.R., Spivak, M., Wilson, W.T. Amitraz resistance in Varroa: new discovery in North America. American Bee Journal. 139(5); 362. 1999.

4.      S. Bogdanov and colleagues, Acaricide Residues in Honey Beeswax and Propolis, 1999, World Wide Web site accessed August 25, 2003 <http://www.apis.admin.ch/english/pdf/BeeProducts/Acaricides_e.pdf>


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